Product Overview
AFFINIX Series
QCM apparatus that quantifies changes in physical properties and intermolecular interactions, the AFFINIX Series.
The AFFINIX Series is a group of apparatus that uses QCM to visualise molecular recognition and the changes caused by subsequent reactions by detecting mass changes at the nano level.
AFFINIX Series
Key points for product selection
| AFFINIX Q8 | AFFINIX QN Pro | AFFINIX QNμ | AFFNIX QN | |
|---|---|---|---|---|
| Installation of a simple and easy interaction analyzer | ○ | ○ | ◎ | ◎ |
| Measurement of coupling constant and rate constant | ◎ | ○ | ○ | ○ |
| Reduction of the consumption of samples | ◎ | ○ | ○ | △ |
| High number of samples to be measured | ◎ | ○ | ○ | ○ |
| Organic solvent present in test solution | △ | △ | △ | ○ |
| Immobilization using organic solvents | ○＊＊ | ○＊ | ○＊ | ◎ |
| Quantitative determination of the viscoelasticity of adsorbates | － | ◎ | － | － |
| Quantitative determination of the viscosity of the solution | － | ◎ | － | － |
| Q8 | QN Pro | QNμ | QN | |
|---|---|---|---|---|
| Installation of a simple and easy interaction analyzer | ○ | ○ | ◎ | ◎ |
| Measurement of coupling constant and rate constant | ◎ | ○ | ○ | ○ |
| Reduction of the consumption of samples | ◎ | ○ | ○ | △ |
| High number of samples to be measured | ◎ | ○ | ○ | ○ |
| Organic solvent present in test solution | △ | △ | △ | ○ |
| Immobilization using organic solvents | ○＊＊ | ○＊ | ○＊ | ◎ |
| Quantitative determination of the viscoelasticity of adsorbates | － | ◎ | － | － |
| Quantitative determination of the viscosity of the solution | － | ◎ | － | － |
＊Please use crystal separated sensor type
＊＊Please remove the measuring cup before use
◎ ： Especially recommended ○ ： Recommended △ ： Not very suitable － ： Unmeasurable
＊Please use crystal separated sensor type
＊＊Please remove the measuring cup before use
◎ ： Especially recommended
○ ： Recommended
△ ： Not very suitable
－ ： Unmeasurable
AFFINIX Series
Applications
Intermolecular Interaction Analysis(Bio and Materials related)
・Quantifying interactions between substances in real-time and label free
・Affinity and kinetics (Kd, Ka, kon, koff) analysis of interactions between molecules
・Measurement of complex multimolecular interactions
・Establishment of experimental conditions and preliminary considerations
・Quantification of bound substance, detection of interactions in crude solution
・Quantification of adsorbed molecules on surface
 |  |  |
| Protein Related | DNA Related | Sugar Chain Related |
| Protein-protein interactions Antigen-antibody reactions β-Amyloid etc. aggregation reactions Analysis of protein complex formation Cofactor search | Hybridisation Detection of mismatched base pair RNA related reactions Transcription factor search Evaluation of antibiotics and hybrid inhibitors | Glyco-lectin interactions Sugar-binding proteins Chitin/chitosan binding |
| [Application notes] Self aggregation of Prion reactions | [Application notes] Analysis of DNA Elogation Reaction DNA-Hybridization-Inhibiting Metal Complex | |
 |  |  |
| Lipid Related | Oxygen Reactions | Binding of Small Molecules |
| Reaction of lipid membrane and antimicrobial peptide Surface treatment and ribosome binding Adsorption of catechins to lipid membrane | Decomposition reaction by Dnase Decomposition reaction by trypsin Extension reaction by DNA polymerase Hydrolysis reaction on sugar chain Sugar chain lengthening reaction | Evaluation of inhibitors Evaluation of drug binding Ligand-receptor interactions Evaluation of bonding between small molecules Evaluation of toxin |
| [Application notes] Adsorption of Liposome to Peptide Binding of Pharmaceutical Small Molecules an T7 Phage | ||
 |  |  |
| Application Examples (Biology) | Special Sensor | Evaluation of Organic Polymers |
| Selection of highly effective oxygen detergent Antibody reagent degradation test Determination of meat maturity Determination of blood type from trace body fluid Selection sensor blocking agent | Metal surface sensor (Ti, Ni, Al, etc.) Metal oxide surface sensor (SiO2 etc.) | Adsorption of microparticles to polymers Adsorption to latex beads Evaluation of polymer solubility Molecular adsorption to biocompatible membranes |
| [Application notes] Detection of Verotixin | ||
 |  |  |
| Evaluation of Inorganic Materials | Atmospheric Measurements | Application Examples (Material) |
| Adsorption to carbon nanotubes Adsorption to fullerene Adsorption to metal nanocolloid Adsorption to hydroxyapatite Evaluation of metal surfaces solubility | Effects of light irradiation treatment Adsorption of smoke particles Odour sensor | Evaluation of dirt adsorption to coating agent Determination of optimum use amount of detergent Evaluation of antifouling agent Dye adsorption on cotton Determination of optimum conditions for substrate fabricatio |
|
| Protein Related |
| Protein-protein interactions Antigen-antibody reactions β-Amyloid etc. aggregation reactions Analysis of protein complex formation Cofactor search |
| [Application notes] Self aggregation of Prion reactions |
|
| DNA Related |
| Hybridisation Detection of mismatched base pair RNA related reactions Transcription factor search Evaluation of antibiotics and hybrid inhibitors |
| [Application notes] Analysis of DNA Elogation Reaction DNA-Hybridization-Inhibiting Metal Complex |
|
| Sugar Chain Related |
| Glyco-lectin interactions Sugar-binding proteins Chitin/chitosan binding |
|
| Lipid Related |
| Reaction of lipid membrane and antimicrobial peptide Surface treatment and ribosome binding Adsorption of catechins to lipid membrane |
| [Application notes] Adsorption of Liposome to Peptide Binding of Pharmaceutical Small Molecules an T7 Phage |
|
| Oxygen Reactions |
| Decomposition reaction by Dnase Decomposition reaction by trypsin Extension reaction by DNA polymerase Hydrolysis reaction on sugar chain Sugar chain lengthening reaction |
|
| Binding of Small Molecules |
| Evaluation of inhibitors Evaluation of drug binding Ligand-receptor interactions Evaluation of bonding between small molecules Evaluation of toxin |
|
| Application Examples (Biology) |
| Selection of highly effective oxygen detergent Antibody reagent degradation test Determination of meat maturity Determination of blood type from trace body fluid Selection sensor blocking agent |
| [Application notes] Detection of Verotixin |
|
| Special Sensor |
| Metal surface sensor (Ti, Ni, Al, etc.) Metal oxide surface sensor (SiO2 etc.) |
|
| Evaluation of Organic Polymers |
| Adsorption of microparticles to polymers Adsorption to latex beads Evaluation of polymer solubility Molecular adsorption to biocompatible membranes |
|
| Evaluation of Inorganic Materials |
| Adsorption to carbon nanotubes Adsorption to fullerene Adsorption to metal nanocolloid Adsorption to hydroxyapatite Evaluation of metal surfaces solubility |
|
| Atmospheric Measurements |
| Effects of light irradiation treatment Adsorption of smoke particles Odour sensor |
|
| Application Examples (Material) |
| Evaluation of dirt adsorption to coating agent Determination of optimum use amount of detergent Evaluation of antifouling agent Dye adsorption on cotton Determination of optimum conditions for substrate fabricatio |
Viscoelasticity Analysis
・Nano level viscoelasticity analysis of the surface or the interface of adsorbed layer
・Calculation of viscoelasticity parameters (G’, G”, tanδ etc.)
・Measurement of viscoelastic changes associated with molecular recognition of biomolecules
・Evaluation of surface physical properties such as swelling and viscoelasticity of adsorption film
・Microviscosity measurement of polymer solutions and high-concentration protein solutions
・Viscoelasticity analysis of hardening, solidification, and phase transition processes of materials
 |  |  |
| Viscoelasticity analysis of adsorbed film | Structural change of adsorption film | Measurement during liquid temperature change |
| Viscoelasticity analysis of protein-protein interaction Viscoelasticity analysis of DNA, sugar-chain and lipid Observation of film hardness change due to binding | Observation of structural change of temperature responsive polymer Observation of structural change of protein Adsorption behaviour of lipids or ribosomes on solid surfaces | Observation of cloud point of surfactant Viscoelasticity observation of biocompatible polymers Monitoring of liquid crystal, gel phase transition |
 |  |  |
| Viscoelasticity analysis | Ultra trace measurement | Separate measurement of mass and viscosity |
| Viscoelasticity analysis of soft material (colloid, macromolecule etc.) Monitoring of gelation process Viscoelasticity analysis of adhesive, resin, grease etc. | Adsorption measurement of rare samples Viscosity measurement of Newtonian fluid Viscoelasticity measurement of high concentration protein | Evaluation of drug dissolved in organic solvent Adsorption measurement of sample dissolved in glycerol Interaction measurement of proteins in crude solutio |
|
| Viscoelasticity analysis of adsorbed film |
| Viscoelasticity analysis of protein-protein interaction Viscoelasticity analysis of DNA, sugar-chain and lipid Observation of film hardness change due to binding |
|
| Structural change of adsorption film |
| Observation of structural change of temperature responsive polymer Observation of structural change of protein Adsorption behaviour of lipids or ribosomes on solid surfaces |
|
| Measurement during liquid temperature change |
| Observation of cloud point of surfactant Viscoelasticity observation of biocompatible polymers Monitoring of liquid crystal, gel phase transition |
|
| Viscoelasticity analysis |
| Viscoelasticity analysis of soft material (colloid, macromolecule etc.) Monitoring of gelation process Viscoelasticity analysis of adhesive, resin, grease etc. |
|
| Ultra trace measurement |
| Adsorption measurement of rare samples Viscosity measurement of Newtonian fluid Viscoelasticity measurement of high concentration protein |
|
| Separate measurement of mass and viscosity |
| Evaluation of drug dissolved in organic solvent Adsorption measurement of sample dissolved in glycerol Interaction measurement of proteins in crude solutio |
AFFINIX Series
Specifications
| Item | AFFINIX QN | AFFINIX QN μ(Micro) | AFFINIX QN Pro | AFFINIX Q8 |
|---|---|---|---|---|
| Model | QCM2008-STKIT | QCM2008-LVKIT | QCM2008-PRKIT | QCM2012(Standard Model)/ QCM2012E(Limited Function Model) |
| Measurement Principle・System | Quartz Crystal Microbalance(QCM) oscillation | Quartz Crystal Microbalance(QCM) oscillation | Quartz Crystal Microbalance (QCM) ・ Admittance Analysis Methods | Quartz Crystal Microbalance(QCM) oscillation |
| Measurement Frequency・Sensiticity | 27MHz(Fundamental frequency) 30pg/Hz | 27MHz(Fundamental frequency) 30pg/Hz | 27MHz (Fundamental) ・ 30pg/Hz 81MHz (Overtone) ・ 10pg/Hz | 27MHz(Fundamental frequency) 30pg/Hz(620pg/cm2・Hz) |
| Dynamic Range | 100pg(per sensor) | 100pg(per sensor) | 100pg～10μg | 100pg～10μg(2ng/cm2～200μg/cm2) |
| Oscillation Stability | Noise ≦ 1 Hz(distilled water, 25℃, at stable state) | Noise ≦ 2 Hz(27MHz, distilled water, 25℃, at stable state) | Noise ≦ 2 Hz(27MHz, distilled water, 25℃, at stable state) | Noise ≦ 1 Hz(27MHz, distilled water, 25℃, at stable state) |
| Average Drift | ≦ 1 Hz/min(distilled water, 25℃, at stable state) | ≦ 1 Hz/min(distilled water, 25℃, at stable state) | ≦ 1 Hz/min (27MHz, distilled water, 25℃, at stable state) | ≦ 1 Hz/min(distilled water, 25℃, at stable state) |
| Injection System | Manual, Multiple injection is possible | Manual, Multiple injection is possible | Manual, Multiple injection is possible | Manual, Multiple injection is possible |
| Min. Injection Volume | 0.1μL～ (dependent on micropipette to be used) | 0.1μL～ (dependent on micropipette to be used) | 0.1μL～ (dependent on micropipette to be used) | 0.1μL～ (dependent on micropipette to be used) |
| Channel Number | 1 | 1 | 1 | 8 |
| Cup Volume | Glass cell[5～11mL]※Consumable mini cell[1.6mL] | 400～550μL | 400～550μL | 80～120μL |
| Sensor Type | Ceramic sensor chip | Sensor cell | Sensor Cup (specific for QN Pro) | Sensor with a removable cup |
| Stir Method | Magnetic Stirrer | Magnetic Stirrer | Magnetic Stirrer | Stirrers driven by motors 0～3,000rpm(setting step：100rpm) 3,000 rpm(fixed) |
| Temp. Control System | Peltier | Peltier | Peltier | Peltier |
| Setting Temperature | 0.1～50.0℃(step：0.1℃) | 10.0～50.0℃(step：0.1℃) | 10.0～60.0℃(step：0.1℃) | 10.0～40.0℃(step：0.1℃) ※Range appliable to standard model only with minimum set temp. 10℃ below room temp. ※※25℃ fixed for Limited Function Model |
| System Software | Measuring software (PC limited license pre-installed in provided PC, there is no installer disc) Install-free data viewer software included by default in CD | Measuring software (PC limited license pre-installed in provided PC, there is no installer disc) Install-free data viewer software included by default in CD | Measuring software：『AFFINIX QN Pro』 (PC limited license pre-installed in provided PC, there is no installer disc) Data viewer software：『AFFINIX QN Pro Viewer』 (The installer is saved in PC and an additional disc) | Measuring software (PC limited license pre-installed in provided PC, there is no installer disc) Install-free data viewer software included by default in CD |
| Date Processing Part | Windows Laptop PC | Windows Laptop PC | Windows Laptop PC | Windows Laptop PC |
| Communication System | Ethernet Virtual serial | Ethernet Virtual serial | Ethernet Virtual serial PC|card | Ethernet Virtual serial |
| Output parameters | - | - | Fs,F2,Fw,Q,D,R,(C0,C1,L1) | - |
| Operating Environment | 20～30℃(Recommended) | 20～30℃(Recommended) | 20～30℃(Recommended) | 20～30℃(Recommended) |
| Size | 140W×350D×220H(mm) | 140W×350D×220H(mm) | 140W×300D×220H(mm) (AFFINIX QN main unit) 71W×360D×144H(mm) (Analysing unit) | 220W×410D×170H(mm) (AFFINIX Q8 main unit) |
| Weight | 7.7kg | 7.1kg | 9.0kg | 7.0kg (Affinix Q8 main unit only) |
| Power Supply | AC 100～240V, 1A | AC 100～240V, 1A | AC 100～240V, 3A (Body：1A, Analyzing Unit：2A) | AC 100～240V, 1A (Affinixx Q8 main unit only) |
| Optional accessory | Data Analysis Software AFFINIX Q User Analysis (AQUA) Installer CD | Data Analysis Software AFFINIX Q User Analysis (AQUA) Installer CD | Data Analysis Software(AQUA) | Data Analysis Software AFFINIX Q User Analysis (AQUA) Installer CD |
| Standard items | AFFINIX QN Base Equipment(1) Software included PC(1) PC accessories(with LAN Cable)(1) Glass cell(1) Stirrer(1) USB memory stick(1) Sensor chips(4 in a case) Instruction Manual(1) Data Viewer(1) | AFFINIX QNμ Base Equipment(1) Software included PC(1) PC accessories(with LAN Cable)(1) Cell cover with stirrer(1) USB memory stick(1) Sensor cells(4 in a case) Instruction Manual(1) Data Viewer(1) | AFFINIX QN Pro Base Equipment(1) Communication / Measurement Kit(1) Measurement Control Software included PC(1) PC accessories(with LAN Cable)(1) Instruction Manual(1) Cell Cover with Stirring Bar(1) USB memory stick(1) Sensor cups(4 pieces/case(1)) Calibration Set(1) Data Viewer(1) | AFFINIX Q8 Base Equipment(1) Software included PC(1) PC accessories(with LAN Cable)(1) Instruction Manual(1) Sensor holder(1) Sensor tray sensor pack(8 in a set) Data Viewer(1) |
| PC Spec. | PC with pre-installed measurement software | PC with pre-installed measurement software | PC with pre-installed measurement software | PC with pre-installed measurement softwa |